The streaming service will show UEFA Champions League matches featuring Italian sides every Wednesday night.
Amazon has won the rights to exclusively broadcast 16 UEFA Champions League matches on Wednesday nights live in Italy, for three seasons from 2021-2024.
The deal also includes the UEFA European Super Cup, which is played by the winning teams of the Champions League and the Europa League.
The Wednesday night UEFA Champions League matches on Amazon will always feature an Italian side during the group stages and, where they have qualified, through to the semi-finals.
An Amazon spokesperson said: “Amazon is looking forward to UEFA Champions League, one of the world’s most prestigious team championships. We are delighted to offer our customers in Italy the Wednesday night top matches from 2021 onwards. We know that Italian football fans are among the most passionate in Europe and we’ll be working hard to deliver a fantastic football experience that brings them close to the action.”